Table 4 Fold changes of significant differential expressed unigenes revealed in auxin-responsive seed dormancy and germination regulation by RNA-seq and their verification by RT-PCR
Gene | Encoded protein | RNA-seq | RT-PCR | ||||||
|---|---|---|---|---|---|---|---|---|---|
Untreated-0 vs. H2O-0 | IAA-0 vs. H2O-0 | H2O-72 vs. IAA-72 | IAA-156 vs. IAA-72 | Untreated-0 vs. H2O-0 | IAA-0 vs. H2O-0 | H2O-72 vs. IAA-72 | IAA-156 vs. IAA-72 | ||
ABA and GA crosstalk | Â | Â | Â | Â | Â | Â | Â | Â | Â |
 TCONS_00064877 | AHG3(Protein phosphatase 2C) | – | 0.318 | – | – | – | 0.3407 | – | – |
 TCONS_00038628 | GA3ox1 | 0.072 | – | 6.848 | – | 0.0146 | – | 1.6385 | – |
 TCONS_00082895 | GA2ox2 | – | – | – | 13.934 | – | – | – | 5.5919 |
 TCONS_00084330 | CYP707A2 | 0.008 | – | – |  | 0.0273 | – | – | – |
ABA and auxin crosstalk | Â | Â | Â | Â | Â | Â | Â | Â | Â |
 TCONS_00033126 | PIN4 | – | – | 10.190 | 5.358 | – | – | 3.9632 | 7.2267 |
Met pathway | Â | Â | Â | Â | Â | Â | Â | Â | Â |
 TCONS_00038414 | Cysteine synthase | 0.094 | 2.415 | – | – | 0.0386 | 2.8547 | – | – |
 TCONS_00035466 | AdoMet:Met S-methyltransferase | – | 0.105 | – | – | – | 0.5236 | – | – |
 TCONS_00000319 | S-adenosylmethionine synthas | – | – | 5.156 | 7.679 | – | – | 1.7983 | 4.4588 |
 TCONS_00049344 | Met synthase | – | – | 4.748 | 5.275 | – | – | 1.9543 | 3.8106 |
 TCONS_00052600 | Serine acetyltransferase | – | – | – | 19.437 | – | – | – | – |
Epigenetic regulators |  |  |  |  |  | – | – | – | – |
 TCONS_00072327 | EFS (Histone H3 methyltransferase) | – | 20.418 | – | – | – | 0.3253 | – | – |
 TCONS_00057450 | HUB1(C3HC4 RING finger) | – | 6.917 | – | – | – | 0.7614 | – | – |