Fig. 5
From: Functional and evolutionary analyses of the miR156 and miR529 families in land plants
Phenotypic and expression analyses of 35S::OsMIR529b Arabidopsis plants. a Morphology of six-week-old (upper pannel) and 25-day-old (lowerpannel) plants (Col-0 or wild type, 35S::MIR156a, spl9;spl15 mutant, 35S::OsMIR529b). Scale bar represents 1 cm. b Stem–loop pulsed RT-PCR to detect OsMIR529b precursor, OsmiR529b, miR156, and AtSPL transcripts in Arabidopsis leaf tissues. Reactions without RT (−RT) and without cDNA (−) were used as negative controls. Numbers between brackets indicate PCR cycles. Actin-2 was used as an internal control. c Modified 5′-RACE analyses of cleaved SPL15 transcripts in Col-0 and 35S::OsMIR529b leaf tissues. The 5′-ends of the SPL15 cleavage products preferentially map to miR156 (in Col-0) and miR529b binding sites (in 35S::OsMIR529b)