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Fig. 1 | BMC Plant Biology

Fig. 1

From: Overexpression of a brassinosteroid biosynthetic gene Dwarf enhances photosynthetic capacity through activation of Calvin cycle enzymes in tomato

Fig. 1

BR biosynthesis capacity in mutant defective in Dwarf (d im), wild type (WT) and Dwarf overexpressing lines (DWF:OX2 and DWF:OX3). a Confirmation for the presence of transgene in the selected lines by PCR. M, 100 bp marker; V+, transformed vector; V-, genomic DNA of WT plants; 1–7, selected plant lines. bd Dwarf transcript and accumulation of BR biosynthetic precursors. Leaves were harvested at 45d after germination for the gene expression and BRs content analysis. Data are the means ± SD of four independent biological samples. Means denoted by the same letter did not differ significantly according to Tukey’s test (P < 0.05). CS, castasterone; 28-norCS, 28-norcastasterone; BL, brassinolide

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