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Fig. 5 | BMC Plant Biology

Fig. 5

From: Two members of TaRLK family confer powdery mildew resistance in common wheat

Fig. 5

The molecular identification of TaRLK1 and TaRLK2 transgenic plants and powdery mildew resistance evaluation. Using specific primer pair, we performed PCR to identify TaRLK1 and TaRLK2 T0 positive transgenic plants (a) and qRT-PCR to study the gene expression level of TaRLK1 and TaRLK2 in T0 positive transgenic plants, “*” indicates significant differences at 0.05 levels, compared with Yangmai 158. Powdery mildew resistance evaluation for TaRLK1 and TaRLK2 T0 transgenic plants at seedling and adult leaf stage were conducted, using susceptible Yangmai 158 as control (c). The hyphae growing on the surface of leaves from transgenic lines for TaRLK2 gene at seedling growing stage (d) and transgenic lines for TaRLK1 and TaRLK2 at adult growing stage (e) were observed, using susceptible Yangmai 158 as control

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