Table 1 Primer pairs for the selected genes and the reference gene
From: Expression profiling of genes involved in drought stress and leaf senescence in juvenile barley
| Gene | Functional annotation | Acc. No. | Primer (FOR and REV) | Ampl. | |
|---|---|---|---|---|---|
| Drought stress genes | A1 | ABA inducible gene |
GenBank: X78205.1 |
ACACGGCGCAGTACACCAAGGAGT CCCACCACGGCGTTCACCAC | 100 bp |
| Dhn1 | Dehydrin 1 |
GenBank: AF181451 |
GCAACAGATCAGCACACTTCCA GCTGACCCTGGTACTCCATTGT | 141 bp | |
| GAD3 | Glutamate decarboxylase 3 |
GenBank: AY187941 |
ATGGAGAACTGCCACGAGAA GGAGATCTCGAACTCGTCGT | 147 bp | |
| NADP_ME | NADP-dependent malic enzyme-like |
GenBank: XM_003569737 |
ATGGCGGGAAGATCAGGG ATCCCTCAGCAGGGAATGC | 165 bp | |
| P5CS2 | Delta 1-pyrroline-5-carboxylate synthase 2 |
GenBank: AK249154.1 |
GTATACATGCACGTGGACCC CAGAGGGTTTTCGCCGAATC | 164 bp | |
| Leaf senescence genes | Contig7437 | SAG senescence associated gene |
GenBank: KF190467.1 |
GCTGAACGGCTGCCACTCCC GAAACCATCGCGCCTGTGGTG | 78 bp |
| GSII | Glutamine synthetase 2 |
GenBank: X53580.1 |
ACGAGCGGAGGTTGACAG CGCCCCACACGAATAGAG | 94 bp | |
| hv_36467 | SAG senescence associated gene |
GenBank: AK367894.1 |
CAGTCCTTTTGCGCAGTTTTC CCAAGCGAGAATGCCTTGTAA | 152 bp | |
| LHC1b20 | Light-harvesting complex I |
GenBank: S68729.1 |
CTGACCAAGGCGGGGCTGATGAAC TCGTGGGGCGGGAGGCTGTAG | 200 bp | |
| pHvNF-Y5α | SAG senescence associated gene |
GenBank: AK370570 |
CATGAAGCGAGCTCGTGGAACA GGTGCGAAGGTGGGACTACTCTGA | 126 bp | |
| Genes out of GWASa | AVP1 | Vacuolar proton-inorganic pyrophosphatase |
GenBank: AY255181.1 |
GACCCTCTCAAGGACACCTC TCCCAACCGGCAAAACTAGA | 160 bp |
| ETFQO | Electron transfer flavoprotein-ubiquinone oxidoreductase |
GenBank: BT000373.1 |
CCACAACCCTTTCTTGAATCCG GATCTAAGGGCGTGGTGAATTT | 160 bp | |
| SAPK9 | Serine/threonine protein |
GenBank: AB125310.1 |
TCATGCAAGACTGTTTCTTGGG TTTCTTCTTGGCACAAAGCATATT | 149 bp | |
| TRIUR3 | Protein kinase | ||||
|
GenBank: M94726 |
ACATTGACGTTGAGAGCAGC GCTACAGAGAATTTGTGACCCA | 151 bp | |||
| HvGAPDH | Glyceraldehyde-3-phosphate dehydrogenase |
GenBank: DQ196027.1 |
CAATGCTAGCTGCACCACCAACTG CTAGCAGCCCTTCCACCTCTCCA | 165 bp |