Table 1 Primer pairs for the selected genes and the reference gene

Drought stress genes

A1

ABA inducible gene

GenBank:

X78205.1

ACACGGCGCAGTACACCAAGGAGT

CCCACCACGGCGTTCACCAC

100 bp

Dhn1

Dehydrin 1

GenBank:

AF181451

GCAACAGATCAGCACACTTCCA

GCTGACCCTGGTACTCCATTGT

141 bp

GAD3

Glutamate decarboxylase 3

GenBank:

AY187941

ATGGAGAACTGCCACGAGAA

GGAGATCTCGAACTCGTCGT

147 bp

NADP_ME

NADP-dependent malic enzyme-like

GenBank:

XM_003569737

ATGGCGGGAAGATCAGGG

ATCCCTCAGCAGGGAATGC

165 bp

P5CS2

Delta 1-pyrroline-5-carboxylate synthase 2

GenBank:

AK249154.1

GTATACATGCACGTGGACCC

CAGAGGGTTTTCGCCGAATC

164 bp

Leaf senescence genes

Contig7437

SAG senescence associated gene

GenBank:

KF190467.1

GCTGAACGGCTGCCACTCCC

GAAACCATCGCGCCTGTGGTG

78 bp

GSII

Glutamine synthetase 2

GenBank:

X53580.1

ACGAGCGGAGGTTGACAG

CGCCCCACACGAATAGAG

94 bp

hv_36467

SAG senescence associated gene

GenBank:

AK367894.1

CAGTCCTTTTGCGCAGTTTTC

CCAAGCGAGAATGCCTTGTAA

152 bp

LHC1b20

Light-harvesting complex I

GenBank:

S68729.1

CTGACCAAGGCGGGGCTGATGAAC

TCGTGGGGCGGGAGGCTGTAG

200 bp

pHvNF-Y5α

SAG senescence associated gene

GenBank:

AK370570

CATGAAGCGAGCTCGTGGAACA

GGTGCGAAGGTGGGACTACTCTGA

126 bp

Genes out of GWAS^a

AVP1

Vacuolar proton-inorganic pyrophosphatase

GenBank:

AY255181.1

GACCCTCTCAAGGACACCTC

TCCCAACCGGCAAAACTAGA

160 bp

ETFQO

Electron transfer flavoprotein-ubiquinone oxidoreductase

GenBank:

BT000373.1

CCACAACCCTTTCTTGAATCCG

GATCTAAGGGCGTGGTGAATTT

160 bp

SAPK9

Serine/threonine protein

GenBank:

AB125310.1

TCATGCAAGACTGTTTCTTGGG

TTTCTTCTTGGCACAAAGCATATT

149 bp

TRIUR3

Protein kinase

GenBank:

M94726

ACATTGACGTTGAGAGCAGC

GCTACAGAGAATTTGTGACCCA

151 bp

HvGAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GenBank:

DQ196027.1

CAATGCTAGCTGCACCACCAACTG

CTAGCAGCCCTTCCACCTCTCCA

165 bp