Fig. 7

Characterization of p35S-CENH3-GFP expression in Arabidopsis plant development. a-g Expression profile and centromere-specific localization of the p35S-CENH3-GFP recombinant protein in the primary root tip a and b, root elongation zone c, root differentiation zone d, the proliferating root tip e and the lateral root tip f and g. a-b Scale bars, 25 μm. c-g Scale bars, 10 μm. h-i Bright field and GFP fluorescence of a p35S-CENH3-GFP root hair. Scale bar, 20 μm. j-k Centromere-specific p35S-CENH3-GFP signals in emerging leaves of 8-day-old seedlings j and mature petals at anthesis k. Scale bars, 10 μm. l-m Frequency distribution of centromeric GFP signals in nuclei of young leaves l and petals m at different stages in development. Error bars represent standard deviation (of three independent analyses of at least 100 spores)