Fig. 4

Conservation of amino acid sequences of Gα and Gγ in Pinaceae and selected angiosperm families. Conservation level of GPA1-like protein sequences in Fabaceae, Brassicaceae, Pinaceae and between the Fabaceae and Brassicaceae (a). Conservation level of long Gγ-subunit/C-type-like protein sequences in Fabaceae, Brassicaceae, Pinaceae and between the Fabaceae and Brassicaceae (b) and amino acid conservation level of short Gγ-subunit-like protein sequences in Fabaceae, Brassicaceae, Pinaceae, Picea, Pinus and between the Fabaceae and Brassicaceae (c). The bar diagram shows the mean amino acid mismatches per protein sequence length in percentage of the Gα-and Gγ-subunits of the Pinaceae, Fabaceae and Brassicaceae estimated in a pairwise sequence comparison within the on the x-axis indicated sequence clusters. Error bars indicate the standard deviation. The heatmap gives the significant differences estimated using one-way ANOVA and the post-hoc Tukey-test. The color scale corresponds to the significance levels and is applied to all heatmaps