Fig. 1
Hyp-O-GALT activity of GALT1-GALT6 transiently expressed in N. tabacum. GALT1-GALT6 were expressed in epidermal cells of tobacco leaves by Agrobacterium-mediated transient expression, which were used for the preparation of Golgi-enriched microsomal membrane proteins for the Hyp-GALT assays. Synthetic peptide [AO]7 was used as substrate acceptor. WT tobacco leaves infiltrated with Agrobacterium GV3101 strain (Empty vector), WT tobacco leaves, and WT tobacco leaves infiltrated with ST fused with GFP were used as controls. Experiments were performed using duplicate samples and data represent the mean ± SD from two independent experiments. Asterisks indicate mean values significantly different from the WT (Dunnett’s test, *P <0.05; **P <0.01)