Fig. 4
From: The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment
Changes in photosynthetic pigments (a chlorohyll a, b chlorophyll b, c chlorophyll a/b, d violaxanthin, e neoxanthin, f lutein, g β-carotene ) in detached Arabidopsis leaf halves either irradiated with UV-B (8 W·m−2) for 5 min or covered with black paper (control) and left either in darkness or under constant white light (100 μmol·m−2·s−1) for the indicated time. Day 0 means 1 h after the treatment. Non-irradiated leaf halves served as a control. Pigments were separated by HPLC with detection by absorbance at 436 nm (chlorophylls) or 405 nm (carotenoids) and their content was determined from the area under the peak of the chromatogram using the extinction coefficients listed in Additional file 2: Table S2. Statistical significance of the differences between treatments was assessed with one-way ANOVA and the results of this analysis are listed in Additional file 3: Table S3