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Fig. 3 | BMC Plant Biology

Fig. 3

From: Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes

Fig. 3

Immunoblot analysis of the N-terminal FLAG-tagged RING protein library expressed by the wheat germ cell-free system. For analysis, 2 μL of crude recombinant RING proteins with N-terminus FLAG tag was loaded onto SDS-PAGE and detected by anti-FLAG-HRP antibody. A total of 204 out of 208 RING proteins analysed were detected. Arrows show the expected signal for each RING protein. Blue asterisks refer to proteins with high molecular smears, while red asterisks refer to RING proteins did not show high molecular smears and were subsequently used in the in vitro ubiquitination analysis (Fig. 5, Fig. 6)

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BMC Plant Biology

ISSN: 1471-2229

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