Fig. 4From: One amino acid makes the difference: the formation of ent-kaurene and 16α-hydroxy-ent-kaurane by diterpene synthases in poplarSensitivity of PtTPS17 ent-CPP formation to Mg2+. The enzyme was expressed in E. coli, extracted, partially purified, and incubated with the substrate GGPP. The product CPP was hydrolyzed using HCl and extracted with hexane and analyzed by GC-MSBack to article page