Fig. 7
Functional analysis of the PpNAC3 promoter in VIGS-silenced NbbHLH2 plants. a Photographs of 6-week-old control (pTRV-control) and NbbHLH2-silenced plants. b The relative expression level of NbbHLH2 was determined by qPCR in pTRV control and N. benthamiana plants with NbbHLH2 suppression. c GFP expression driven by the PpNAC3 promoter in pTRV-NbbHLH2-silenced plants. After 48 h of agroinfiltration with the PNAC3-GFP construct, the leaves were treated with either 100 μM MeJA or with water as a control. NbActin was used as the endogenous qPCR control. The error bars represent the SD from three biological replicates measured in triplicate