Fig. 3

Amplification and sequencing of CsaMLO8 from genomic DNA isolated from the resistant genotype reveals the insertion of an 1449 bp long Transposable Element (TE). a The genomic region of CsaMLO8 in which deletions in the coding sequence were observed in the resistant genotype was amplified from DNA isolated from both the susceptible and resistant genotypes. Amplified products were analysed on 1.25 % agarose gel. Whereas the product amplified from the susceptible genotype was of the expected size, the product amplified from the resistant genotype was larger than expected. b The product amplified from the resistant genotype as described in (A) was sequenced, which revealed an insertion with a length of 1449 bp. A dot-plot was made of the insertion to see whether the sequence contains repetitive elements. c The first and last 200 bp of the insertion, plus 15 bp of CsaMLO8 before and after the insertion were aligned to one another, to verify the presence of long terminal repeats (LTRs). Non-aligned parts of the sequence are highlighted in red. It can be seen that the first 184 bp of the insertion are completely identical to the last 184 bp of the insertion. There is a duplication of 5 bp from CsaMLO8 before and after the insertion (Target site duplication, 5′-ATTAT-3′). d Schematic representation of the insertion. The locations of LTRs and the 3′ TSD are indicated