Fig. 3From: Low temperature-induced DNA hypermethylation attenuates expression of RhAG, an AGAMOUS homolog, and increases petal number in rose (Rosa hybrida)Silencing of RhAG in rose flowers. Three month old rose plants were infiltrated with A.tumefaciens containing a TRV control (TRV, pTRV1 + pTRV2), or TRV carrying an RhAG fragment (TRV-RhAG, pTRV1 + pTRV2- RhAG). a Phenotype of the TRV control (left) and RhAG-silenced (right) flowers at stage 10. b Quantitative RT-PCR analysis of RhAG expression in TRV control and RhAG-silenced petals. The expression level of RhAG in the TRV19 control was set to 1.0. RhTCTP was used as the internal control. Floral buds at stage 3 were used. Values are means ± SD (n = 3). c Petals from TRV flowers (left) and RhAG-silenced flowers (right). d Petal (left) and stamen (right) numbers of TRV flowers and RhAG-silenced flowers (n = 5). Asterisks indicate significant differences calculated using the t test (**p < 0.01; *p < 0.05). Scale bars = 1 cmBack to article page