Fig. 2

Overexpressing PEX5 but not PEX7 worsens the peroxisomal defects of pex4-1. a, b Seedlings were grown as in the legend of Fig. 1. Means of normalized dark-grown hypocotyl lengths and standard deviations of the means are shown (n ≥ 18). PEX5 was overexpressed in wild type and pex4-1 using the 35S:PEX5 construct [17]. PEX7 was overexpressed in wild type using the 35S:PEX7a construct [27] and in pex4-1 using the 35S:PEX7 construct [25]. c Protein extracts of dark-grown seedlings from 0.5 % sucrose-supplemented plant nutrient media were processed for immunoblotting. The membrane was serially probed with the indicated antibodies. Thiolase is synthesized as a PTS2-containing precursor (p) and cleaved in the peroxisome into a mature (m) form. HSC70 was used to monitor protein loading. The positions of molecular mass markers (in kDa) are indicated on the left. Band intensities were quantified using ImageJ; levels of PEX5 were normalized to the corresponding HSC70 band prior to normalizing to the 22 °C wild-type band to give the listed numbers