Fig. 1

High temperature ameliorates physiological defects and reduces PEX5 levels of pex4-1. Physiological consequences of growth temperature on pex mutants. Seedlings were grown in the dark at 22 or 28 °C with or without 0.5 % sucrose (a), or on media containing 0.5 % sucrose with or without 30 μM IBA (c), 1.2 μM IAA (d), 2 μM 2,4-DB (e), or 600 nM 2,4-D (f). Dark-grown hypocotyl lengths were normalized to the corresponding mean of 0.5 % sucrose treatment. Means of normalized dark-grown hypocotyl lengths and standard deviations of the means are shown (n ≥ 17 for panel A and n ≥ 12 for panels C-F). b Protein extracts of dark-grown seedlings from 0.5 % sucrose-supplemented plant nutrient media in panel (a) were processed for immunoblotting. The membrane was serially probed with indicated antibodies. HSC70 was used to monitor protein loading. The positions of molecular mass markers (in kDa) are indicated on the left. Band intensities were quantified using ImageJ; levels of PEX5 or PEX4 were normalized to the corresponding HSC70 band prior to normalizing to the 22 °C wild-type band to give the listed numbers