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Fig. 5 | BMC Plant Biology

Fig. 5

From: Triterpenoid profiling and functional characterization of the initial genes involved in isoprenoid biosynthesis in neem (Azadirachta indica)

Fig. 5

Total ion chromatograms (TICs) of AiGDS, AiFDS and AiSQS assays and relative expression level of AiSQS; (a) TICs of AiGDS assays; (1) Standard Nerol, (2) Standard geraniol, (3) Co-injection of standard nerol and geraniol, (4) Substrate control, (5) Enzyme control, (6) AiGDS enzyme assay with IPP and DMAPP as substrates, (7) Co-injection of standard geraniol with AiGDS enzyme assay extract. b TICs of AiFDS assays; (1) Standard (E,E)-farnesol, (2) IPP and DMAPP substrate control, (3) Enzyme control, (4) AiFDS enzyme assay with IPP and DMAPP as substrates, (5) Co-injection of standard (E,E)-farnesol and extract of AiFDS enzyme assay with IPP and DMAPP as substrates, (6) Extract of AiFDS enzyme assay with GPP and IPP as substrates. c TICs of AiSQS assays; (1) Standard squalene, (2) Substrate control, (3) Enzyme control, (4) Extract of full length AiSQS enzyme assay with FPP as substrate and NADPH as co-factor, (5) Co-injection of standard squalene and AiSQS enzyme assay extract, (6) Extract of truncated AiSQS enzyme assay with FPP as substrate and NADPH as co-factor and (7) Co-injection of standard squalene and truncated AiSQS enzyme assay extract

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BMC Plant Biology

ISSN: 1471-2229

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