Fig. 2

hsr8-1 sugar hypersensitive phenotypes are suppressed by the pft1-2 mutation. a Identification by microarray analysis of a cluster of six genes that are down regulated in the suppressor line soh715hsr8-1 compared to hsr8-1. Values on the Y-axis are those obtained after normalization of the entire microarray data set. Dark grey bars and light grey bars represent values obtained for the hsr8-1 mutant and the hsr8-1soh715 suppressor line respectively. b Sugar hypersensitive dark development of Col, hsr8-1, soh715hsr8-1 and soh715hsr8-1 complemented with each of the 6 genes of the deletion. Seedlings were grown vertically in the dark for 14 days on MS medium containing 1 % Glucose. Only the genomic fragment containing the At1g25540 gene rescued the dark development phenotype. c Sugar hypersensitive dark development of Col, hsr8-1, the double mutant hsr8-1pft1-2 and pft1-2. Seedlings were grown as described in (B) above. d Quantitative Real-time PCR analysis of β-Amylase mRNA levels in Col, hsr8-1 and the double mutant hsr8-1pft1-2 in response to glucose. Seedlings were grown on MS medium supplemented with 0.5 % glucose in constant light. After 7 days, the seedlings were transferred for 24 h to MS glucose-free liquid medium and then treated for 6 h with MS medium containing 3 % glucose. Errors bars represent SD from three biological replicates. Data shown is representative of three independent experiments. **, p < 0.01 comparing Col to hsr8-1; ***, p < 0.001 comparing hsr8-1 to hsr8-1 pft1-2 (Student’s t- test). Relative transcript levels (RTL) were calculated using transcript levels of the reference gene TUB6 (At5g12250). e Anthocyanin accumulation in response to glucose in Col, hsr8-1 and the double mutant hsr8-1pft1-2. Seedlings were grown in continuous light for 7 days on MS medium containing 1 % glucose (solid bars) or 3 % glucose (dashed bars). Errors bars represent SD from three biological replicates. Data shown is representative of two independent experiments. **, p < 0.01 comparing Col to hsr8-1; ***, p < 0.001 comparing hsr8-1 to hsr8-1 pft1-1 (Student’s t- test). f Quantitative Real-time PCR analysis of the sugar-responsive APL3 gene mRNA levels in Col, hsr3, pft1-2hsr3, hsr4, pft1-2hsr4 and pft1-2 in response to glucose. Hsr3 and hsr4 are sugar-hypersensitive mutations in subunits of the ARP2/3 complex [18]. Seedlings were grown on MS medium supplemented with 0.5 % glucose in constant light. After 7 days, the seedlings were transferred to glucose-free liquid MS medium for 24 h and then treated for 6 h with MS medium containing either 0 % glucose (solid bars) or 3 % glucose (dashed bars). Errors bars represent SD from three biological replicates. **, p < 0.01 comparing Col to hsr3 and Col to hsr4; ***, p < 0.001 comparing hsr3 to hsr3 pft1-2 and hsr4 to hsr4 pft1-2 (Student’s t- test). Relative transcript levels (RTL) were calculated using transcript levels of the reference gene TUB6 (At5g12250)