Table 4 Transcription levels of genes involved in the biosynthesis of catechins, theanine and caffeine
Pathway | Enzyme | Gene # | Tea buds | Tea leaves | Gene # | Oil tea buds | Oil tea leaves | Max.Log10(Tea FPKM)-Max. Log10(Oil Tea FPKM | ||
|---|---|---|---|---|---|---|---|---|---|---|
Description | Abbr. | Max. Log10 (FPKM) | Max. Log10 (FPKM) | Max. Log10 (FPKM) | Max. Log10 (FPKM) | Bud | 2nd leaf | |||
Catechines | phenylalanine ammonia-lyase | PAL | 21 | 6.12 | 5.71 | 20 | 5.22 | 5.01 | 0.90 | 0.70 |
cinnamic acid 4-hydroxylase | C4H | _ | _ | _ | _ | _ | _ | _ | _ | |
4-coumarate--CoA ligase | 4CL | 16 | 5.60 | 5.61 | 17 | 5.60 | 5.82 | 0.00 | −0.21 | |
chalcone synthase | CHS | 37 | 7.27 | 6.90 | 36 | 7.59 | 7.22 | −0.32 | −0.32 | |
chalcone isomerase | CHI | 1 | 5.34 | 4.83 | 1 | 5.68 | 5.27 | −0.34 | −0.44 | |
flavanone 3-hydroxylase | F3H | 5 | 7.18 | 6.61 | 6 | 5.70 | 5.66 | 1.48 | 0.95 | |
flavonoid 3',5'-hydroxylase | F3'5'H | 5 | 6.10 | 5.75 | 4 | 5.40 | 4.90 | 0.70 | 0.85 | |
flavonoid 3'-hydroxylase | F3'H | _ | _ | _ | _ | _ | _ | _ | _ | |
leucoanthocyanidin reductase | LAR | 4 | 6.93 | 7.15 | 6 | 5.10 | 6.33 | 1.83 | 0.82 | |
anthocyanidin synthase | ANS | _ | _ | _ | _ | _ | _ | _ | _ | |
anthocyanidin reductase | ANR | 5 | 6.97 | 6.46 | 2 | 6.55 | 6.09 | 0.42 | 0.37 | |
flavone synthase | FNS | _ | _ | _ | _ | _ | _ | _ | _ | |
dihydroflavonol 4-reductase | DFR | 11 | 4.10 | 3.79 | 10 | 3.68 | 3.52 | 0.42 | 0.27 | |
flavonol synthase | FLS | 12 | 3.43 | 3.14 | 8 | 4.08 | 4.23 | −0.65 | −1.09 | |
Theanine | glutamate synthase | GOGAT | 36 | 3.43 | 3.73 | 34 | 3.95 | 3.98 | −0.52 | −0.25 |
glutamate dehydrogenase | GDH | 9 | 2.83 | 2.55 | 8 | 2.87 | 3.12 | −0.04 | −0.57 | |
alanine aminotransferase | ALT | 1 | 3.47 | 3.05 | 3 | 0.00 | 0.00 | 3.47 | 3.05 | |
glutamine synthetase | GS | 5 | 6.54 | 5.76 | 7 | 5.72 | 6.18 | 0.82 | −0.42 | |
theanine synthetase | TS | _ | _ | _ | _ | _ | _ | _ | _ | |
Caffeine | 5'-nucleotidase | 5'-Nase | 4 | 3.95 | 3.60 | 5 | 4.12 | 3.76 | −0.17 | −0.16 |
IMP dehydrogenase | IMPDH | 1 | 5.26 | 4.95 | 1 | 3.83 | 3.69 | 1.43 | 1.26 | |
ribokinase | RBK | 11 | 4.08 | 4.24 | 13 | 5.18 | 5.02 | −1.10 | −0.78 | |
caffeine synthase | TCS | 2 | 6.63 | 6.34 | 1 | 2.56 | 2.49 | 4.07 | 3.85 | |