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Table 4 The enzymes involved in metabolite biosynthesis were identified in the assembled genome and these genes were analyzed for their expression level in the transcriptome. The RKPM value signifies the level for expression

From: Genome sequencing of herb Tulsi (Ocimum tenuiflorum) unravels key genes behind its strong medicinal properties

Sno. Metabolite Disease implication Enzymes involved No. of hits in Ocimum genome RPKM value
  Flavonoids     
1 Apigenin Anti-cancer Flavone synthaseI 16 -
    Naringenin, NADPH oxygen oxidoreductase 17 -
2 Luteolin Anti-cancer Flavone synthaseI 7 34.44
    Naringenin, NADPH oxygen oxidoreductase 9 61.34
    Flavone 3’ monooxygenase 5 5.53
    Naringenin 3’ hydroxylase 26 104.24
    Eriodictoyl NADPH oxygen oxidoreductase 7 59.79
  Phenylpropanoids     
3 Rosmarinic acid Pathway1 Anti-cancer, anti-oxidant 4-coumaroyl-4’-hydroxyphenyllactate 3-hydroxylase 19 120.52
    Tyrosine_transaminase 2 0.00
4 Rosmarinic acid Pathway 2 Anti-cancer, anti-oxidant Hydroxyphenylpyruvate_reductase 3 45.78
    tyrosine -3-monooxygenase 0 35.58
5 Eugenol Anti-infective Alcohol_o_acetyltransferase 10 4.69
    Eugenol synthase 5 68.93
    Isoeugenol synthase 5 68.93
6 Methylchavicol Anti-fungal, antiparasitic, anti-oxidant Eugenol-o-methyltransferase 4 132.39
  Terpenes     
7 Citral Antiseptic Geraniol synthase 9 0.00
    Geraniol_dehdrogenase 32 46.10
8 Linalool Anti-infective Farnesyl-pyrophosphate synthase 6 128.35
    r-linool_synthase 15 132.39
    s-linool_synthase 22 32.91
  Sesquiterpenes     
9 Caryophylene Anti-inflammatory Alpha humulene synthase 27 3.92
    Beta-caryophyllene synthase 17 3.92
10 Selinene Anti-oxidant Alpha_selinene_synthase 11 13.31
    Beta_selinene_synthase 13  
11 Taxol Anti-cancer Taxadiene synthase 3 9.36
    Taxadiene 5-alpha hydroxylase 33 3.43
    Taxadien-5-alpha-ol O-acetyltransferase 6 26.88
    Taxane 10-beta-hydroxylase 41 3.43
    Taxoid 14-beta-hydroxylase 22 3.43
    2-alpha-hydroxytaxane 2-O-benzoyltransferase 3  
    10-deacetylbaccatin III 10-O-acetyltransferase 3  
    3’-N-debenzoyl-2’-deoxytaxol N-benzoyltransferase 5 4.85
12 Ursolic acid Anti-cancer Cytochrome P450 monooxygenase 23 3.43
13 Oleanolic acid Anti-cancer Beta-amyrin synthase 7 12.69
    Cytochrome P450 monooxygenase 23 3.43
  Sterols     
14 Sitosterol Anti-cancer 24C_methyltransferase 1 67.25
  1. -refers to cases where there was no significant transcript evidence