Multiple interval QTL mapping and searching for PSTOL1 homologs associated with root morphology, biomass accumulation and phosphorus content in maize seedlings under low-P

Table 3 Quantitative trait loci (QTLs) identified using single trait-multiple interval mapping analysis for root length (RL), root diameter (RD), surface area of fine roots (SA2) and root:shoot ratio (R:S) under low-P

Trait	QTL^a	Bin	cM^b	Marker / Position (Mbp)	LOD	Flanking Markers^c / Position (Mbp)		R² (%)^d	Effect^e	R² _T (%)^f
RL	qRL8.05	8.05	100.4	PZA00766_1	2.24	PHM934_19	ZmPSTOL8.05_1	6.87	−0.271**	6.87
				133.8		116.8	152.0
RD	qRD1.03	1.03	94.4	umc1073	3.80	bnlg1083	PZA03742_1	9.60	0.307***	25.64
				32.9		27.5	44.5
	qRD4.05	4.05	25.0	ZmPSTOL4.05	2.74	PHM15427_11	PHM3587-6	6.84	0.270***
				39.8		33.9	59.4
	qRD7.02	7.02	76.0	PZA01690_7	3.95	PZA01933_3	PZA01946_7	10.01	−0.331***
				123.1		98.1	123.6
SA2	qSA2_10.03	10.03	34.2	PHM2770_19	5.16	PHM1155_14	PZA01877_2	15.12	−0.393***	15.12
				72.6		62.1	77.5
R:S	qRS1.07	1.07	206.0	PHM12693_8	3.76	PZA01963_15	PZA03301_2	10.85	0.377***	16.47
				223.5		203.7	240.6
	qRS3.06	3.06	132.0	PZA02212_1	3.00	PZA00186_4	PZA01154_1	8.53	0.310***
				174.5		165.8	216.0

^aQTLs are named using the trait initials followed by their genomic position in bin
^bcM and Mbp indicate the marker position in centiMorgans and in mega base pairs at maximum LOD value
^cFlanking markers are based on −1.5 LOD support interval
^dR²: Ratio of the genotypic variance of the QTL effect to the phenotypic variance, times 100
^eEffects measured as standard deviation from the progeny mean: Positive values indicate that L3 carries the allele for an increase in the trait, and negative values indicate that L22 contributes the allele for an increase in the trait. Effect significance based on p-values estimated via score statistics resampling (⁺ p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001)
^fR² _T: genotypic variance of the full model

ISSN: 1471-2229