Fig. 11

Sl3-MMP was targeted to plasma membrane in onion epidermal cells. Plasmids pFGC-Sl3-MMP and pFGC-eGFP alone were introduced into onion epidermal cells by particle bombardment and fluorescence was observed before (a) and after (b) plasmolysis. Plasmolysis was achieved by treating the epidermal cells with 0.8 M mannitol. Same cells were viewed simultaneously under fluorescent field for GFP and bright field for the intact cells. Merged images of confocal GFP fluorescence and bright field are also presented. Blue arrows indicate the cells undergoing plasmolysis and red arrows indicate the GFP signals on plasma membrane or in cells after plasmolysis