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Fig. 3 | BMC Plant Biology

Fig. 3

From: The maize cytochrome P450 CYP79A61 produces phenylacetaldoxime and indole-3-acetaldoxime in heterologous systems and might contribute to plant defense and auxin formation

Fig. 3

Catalytic activity of CYP79A61. Yeast microsomes containing the heterologously-expressed enzyme a or an empty vector control b were prepared and incubated with the potential substrates L-phenylalanine and L-tryptophan. Products were detected using LC-MS/MS analysis with multiple reaction monitoring in the positive mode. Diagnostic reactions for each product: phenylacetaldoxime, m/z 136.0/119.0; indole-3-acetaldoxime, m/z 175.0/158.0. The structures of all detected CYP79A61 products are shown in c

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BMC Plant Biology

ISSN: 1471-2229

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