Table 2 Analysis of transcript abundance changes of genes associated with the biosynthesis of defence-related metabolites
Transcript ID | Best Ath hit | Gene symbol | Annotation | UV | Ag + | Fold change log2 (UV/n.i.) | FDR-p-value test | Fold change log2 (Ag/n.i.) | FDR-p-value test | RNAseq Unique reads | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
n.i. | UV | AgNO3 | ||||||||||
Tryptophan biosynthesis | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
Thhalv10013041m | AT5G05730.1 | ASA1,TRP5,WEI2 | anthranilate synthase alpha subunit 1 | up | up | 4,83 | 0,000 | 5,31 | 0,000 | 431 | 20815 | 28637 |
Thhalv10010558m | AT3G54640.1 | TRP3,TSA1 | tryptophan synthase alpha chain | up | up | 4,34 | 0,000 | 3,76 | 0,000 | 264 | 9972 | 9692 |
Thhalv10013439m | AT4G27070.1 | TSB2 | tryptophan synthase beta-subunit 2 | up | up | 4,07 | 0,000 | 3,13 | 0,000 | 710 | 3153 | 2355 |
Thhalv10025097m | AT4G27070.1 | TSB2 | tryptophan synthase beta-subunit 2 | up | up | 3,37 | 0,000 | 2,47 | 0,000 | 2187 | 7481 | 5464 |
Thhalv10013857m | AT5G17990.1 | PAT1,TRP1 | tryptophan biosynthesis 1 | up | up | 2,26 | 0,000 | 3,26 | 0,000 | 22 | 207 | 268 |
Thhalv10014630m | AT4G27070.1 | TSB2 | tryptophan synthase beta-subunit 2 | up | up | 1,67 | 0,002 | 1,11 | 0,002 | 2 | 35 | 17 |
Thhalv10002557m | AT2G04400.1 | IGPS | indole-3-glycerol phosphate synthase | up | up | 1,16 | 0,000 | 1,40 | 0,000 | 684 | 6529 | 8538 |
Thhalv10016377m | AT2G29690.1 | ASA2 | anthranilate synthase 2 |  | down | −0,20 | 0,356 | −0,43 | 0,004 | 482 | 436 | 449 |
Thhalv10027732m | AT5G38530.1 | TSBtype2 | tryptophan synthase beta type 2 | down |  | −1,47 | 0,000 | −1,40 | 0,000 | 876 | 407 | 1012 |
Biosynthesis of aliphatic glucosinolates | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | ||
Thhalv10023453m | AT1G62570.1 | FMO GS-OX4 | glucosinolate S-oxygenase 4 | up | up | 4,12 | 0,001 | 3,92 | 0,001 | 243 | 8147 | 7570 |
Thhalv10007582m | AT1G12140.1 | FMO GS-OX5 | glucosinolate S-oxygenase 5 | up | up | 2,12 | 0,000 | 1,48 | 0,000 | 604 | 1102 | 1077 |
Thhalv10018813m | AT1G74090.1 | ATST5B,SOT18 | desulfo-glucosinolate sulfotransf. 18 | Â | Â | 2,12 | 0,000 | 1,75 | 0,000 | 1133 | 461 | 175 |
Thhalv10007073m | AT1G18500.1 | IPMS1,MAML-4 | methylthioalkylmalate synthase-like 4 | Â | 0,31 | 0,084 | 0,32 | 0,165 | 1695 | 1815 | 2672 | |
Thhalv10004037m | AT5G23010.1 | IMS3,MAM1 | methylthioalkylmalate synthase 1 |  | down | −0,60 | 0,055 | −2,92 | 0,000 | 51 | 1 | 4 |
Thhalv10017125m | AT2G43100.1 | ATLEUD1,IPMI2 | isopropylmalate isomerase 2 | down |  | −0,97 | 0,002 | −1,22 | 0,003 | 655 | 462 | 942 |
Thhalv10013695m | AT5G14200.1 | IMD1 | isopropylmalate dehydrogenase 1 | down | down | −1,97 | 0,000 | −1,91 | 0,000 | 1667 | 266 | 779 |
Thhalv10028851m | AT4G12030.2 | BASS5,BAT5 | bile acid transporter 5 | down | down | −2,05 | 0,007 | −4,56 | 0,000 | 11 | 3 | 2 |
Thhalv10024982m | AT4G13770.1 | CYP83A1,REF2 | cytochrome P450 83A1 | down | down | −2,79 | 0,000 | −3,97 | 0,000 | 20919 | 1569 | 2903 |
Thhalv10007301m | AT1G16410.1 | CYP79F1 | cytochrome P450 79 F1 | down | down | −2,92 | 0,005 | −5,72 | 0,000 | 15712 | 1101 | 2740 |
Thhalv10013952m | AT5G07690.1 | MYB29 | myb domain protein 29 | down | down | −3,22 | 0,002 | −4,35 | 0,000 | 3421 | 380 | 61 |
Thhalv10004406m | AT5G61420.2 | MYB28,HAG1 | myb domain protein 28 | down | down | −5,60 | 0,000 | −6,10 | 0,001 | 427 | 10 | 10 |
Indole and general glucosinolate biosynthesis | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | ||
Thhalv10007957m | AT1G21100.1 | IGMT1 | O-methyltransferase family protein | up | up | 5,97 | 0,000 | 4,54 | 0,001 | 165 | 10234 | 6500 |
Thhalv10000114m | AT2G22330.1 | CYP79B3 | cytochrome P450 79B3 | up | up | 5,92 | 0,000 | 5,05 | 0,000 | 16 | 7685 | 4205 |
Thhalv10008152m | AT1G18570.1 | AtMYB51,HIG1 | myb domain protein 51 | up | up | 5,68 | 0,000 | 4,31 | 0,000 | 164 | 13093 | 7845 |
Thhalv10024861m | AT4G39950.1 | CYP79B2 | cytochrome P450 79B2 | up | up | 5,06 | 0,000 | 5,38 | 0,000 | 254 | 24763 | 30609 |
Thhalv10007964m | AT1G21120.1 | IGMT2 | O-methyltransferase family protein | up | up | 4,93 | 0,000 | 2,70 | 0,001 | 434 | 9009 | 9298 |
Thhalv10018795m | AT1G74100.1 | ATST5A,SOT16 | sulfotransferase 16 | up | up | 4,54 | 0,000 | 4,45 | 0,000 | 665 | 26889 | 29842 |
Thhalv10024979m | AT4G37410.1 | CYP81F4 | cytochrome P450 81Â F4 | up | up | 4,52 | 0,000 | 5,27 | 0,000 | 300 | 21717 | 48198 |
Thhalv10008073m | AT1G18590.1 | ATST5C,SOT17 | sulfotransferase 17 | up | up | 4,07 | 0,000 | 2,59 | 0,002 | 877 | 8651 | 4339 |
Thhalv10026067m | AT4G30530.1 | GGP1 | gammaglutamyl peptidase 1 | up | up | 3,31 | 0,000 | 3,55 | 0,000 | 5315 | 66528 | 98213 |
Thhalv10001994m | AT2G20610.1 | SUR1,ALF1,RTY1 | superroot1 | up | up | 3,22 | 0,000 | 2,48 | 0,000 | 14 | 28 | 23 |
Thhalv10018739m | AT1G76790.1 | IGMT5 | O-methyltransferase family protein | up | up | 2,63 | 0,001 | 3,03 | 0,000 | 1747 | 42057 | 94252 |
Thhalv10007574m | AT1G24100.1 | UGT74B1 | UDP-glucosyl transferase 74B1 | up | up | 2,44 | 0,000 | 2,15 | 0,000 | 1062 | 6408 | 5926 |
Thhalv10024981m | AT4G37430.1 | CYP81F1 | cytochrome P450 81Â F1 | Â | up | 1,99 | 0,002 | 4,94 | 0,000 | 131 | 84 | 393 |
Thhalv10004064m | AT4G31500.1 | CYP83B1,SUR2 | cytochrome P450 83B1 | up | up | 1,70 | 0,001 | 2,06 | 0,000 | 2841 | 51916 | 96397 |
Thhalv10027443m | AT4G37400.1 | CYP81F3 | cytochrome P450 81 F3 |  | up | −3,97 | 0,000 | 1,14 | 0,043 | 5 | 9 | 544 |
Phenylpropanoid biosynthesis | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
Thhalv10025563m | AT4G34230.1 | CAD5 | cinnamyl alcohol dehydrogenase 5 | up | up | 4,90 | 0,000 | 4,92 | 0,000 | 590 | 14915 | 11374 |
Thhalv10016314m | AT2G37040.1 | PAL1 | PHE ammonia lyase 1 | up | up | 4,54 | 0,000 | 4,32 | 0,000 | 5639 | 43919 | 53026 |
Thhalv10010153m | AT3G53260.1 | ATPAL2,PAL2 | PHE ammonia lyase 2 | up | up | 4,47 | 0,000 | 4,08 | 0,000 | 5516 | 24942 | 29448 |
Thhalv10016545m | AT2G30490.1 | C4H,CYP73A5 | cinnamate-4-hydroxylase | up | up | 4,47 | 0,000 | 3,92 | 0,000 | 3465 | 40914 | 24286 |
Thhalv10018849m | AT1G80820.1 | CCR2 | cinnamoyl coa reductase | up | up | 4,38 | 0,000 | 3,95 | 0,000 | 9 | 2796 | 6118 |
Thhalv10016544m | AT2G30490.1 | CYP73A5,REF3 | cinnamate-4-hydroxylase | up | up | 4,34 | 0,000 | 4,28 | 0,000 | 200 | 5351 | 6505 |
Thhalv10020406m | AT3G21230.1 | 4CL5 | 4-coumarate:CoA ligase 5 | up | up | 2,86 | 0,000 | 1,30 | 0,000 | 160 | 961 | 882 |
Thhalv10001440m | AT2G43820.1 | SGT1,UGT74F2 | UDP-glucosyltransferase 74Â F2 | up | up | 2,58 | 0,001 | 4,45 | 0,000 | 98 | 1100 | 1193 |
Thhalv10004662m | AT5G08640.1 | FLS1 | flavonol synthase 1 | up | up | 2,41 | 0,003 | 0,80 | 0,026 | 52 | 416 | 498 |
Thhalv10016538m | AT2G40890.1 | CYP98A3 | cytochrome P450, 98A3 | Â | Â | 2,32 | 0,001 | 2,10 | 0,000 | 1482 | 847 | 699 |
Thhalv10011357m | AT1G51680.3 | 4CL1 | 4-coumarate:CoA ligase 1 | up | up | 1,85 | 0,002 | 1,35 | 0,000 | 875 | 3071 | 2269 |
Thhalv10010658m | AT3G55120.1 | A11,CFI,TT5 | Chalcone-flavanone isomerase | up | up | 1,76 | 0,001 | 2,04 | 0,000 | 112 | 352 | 573 |
Thhalv10024928m | AT4G36220.1 | CYP84A1,FAH1 | ferulic acid 5-hydroxylase 1 | up | up | 1,51 | 0,002 | 1,83 | 0,000 | 5064 | 5573 | 8972 |
Thhalv10026028m | AT4G34050.1 | CCoAOMT1 | SAM-dependent methyltransferase | up | Â | 0,99 | 0,010 | 0,04 | 0,779 | 851 | 3093 | 3791 |
Thhalv10000324m | AT3G21230.1 | 4CL5 | 4-coumarate:CoA ligase 5 | Â | up | 0,85 | 0,021 | 0,75 | 0,001 | 28 | 16 | 240 |
Thhalv10008111m | AT1G15950.1 | CCR1 | cinnamoyl coa reductase 1 | up | Â | 0,76 | 0,008 | 0,15 | 0,447 | 423 | 586 | 375 |
Thhalv10000513m | AT3G21230.1 | 4CL5 | 4-coumarate:CoA ligase 5 |  |  | 0,27 | 0,533 | −0,03 | 0,957 | 21 | 17 | 339 |
Thhalv10018769m | AT1G72680.1 | CAD1 | cinnamyl-alcohol dehydrogenase |  | down | −0,15 | 0,461 | −0,23 | 0,050 | 859 | 635 | 666 |
Thhalv10022462m | AT1G65060.1 | 4CL3 | 4-coumarate:CoA ligase 3 |  | down | −0,25 | 0,141 | −0,55 | 0,004 | 113 | 23 | 15 |
Thhalv10020439m | AT3G21230.1 | 4CL5 | 4-coumarate:CoA ligase 5 | down | down | −0,83 | 0,015 | −0,93 | 0,004 | 910 | 214 | 484 |
Thhalv10027317m | AT4G36220.1 | CYP84A1,FAH1 | ferulic acid 5-hydroxylase 1 |  |  | −0,98 | 0,338 | −1,38 | 0,170 | 1 | 1 | 3 |
Thhalv10013289m | AT5G07990.1 | CYP75B1,TT7 | cytochrome P450, 75B1 |  |  | −1,26 | 0,080 | −1,16 | 0,084 | 4 | 2 | 33 |
Thhalv10004668m | AT5G08640.1 | ATFLS1,FLS,FLS1 | flavonol synthase 1 | down | down | −1,43 | 0,005 | −2,38 | 0,002 | 137 | 17 | 41 |
Thhalv10005442m | AT1G43620.1 | TT15,UGT80B1 | UDP-Glycosyltransferase 80B1 | down | up | −1,72 | 0,000 | 0,34 | 0,008 | 1061 | 827 | 4174 |
Thhalv10014054m | AT5G08640.1 | FLS1 | flavonol synthase 1 | down |  | −2,67 | 0,000 | −2,92 | 0,001 | 17 | 11 | 19 |
Thhalv10013745m | AT5G13930.1 | CHS,TT4 | Chalcone and stilbene synth. Fam. | down | down | −4,04 | 0,000 | −3,32 | 0,005 | 214 | 11 | 200 |