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Table 2 Details of 15 genes and the housekeeping gene Actin which were studied with qRT-PCR in seedling leaves harvested from the greenhouse trial in the 509 B. napus inbreds

From: Seedling development traits in Brassica napus examined by gene expression analysis and association mapping

Abb. a Gene name Amplicon size Organism b Reference Start position Primer sequence No. of qRT-PCR
      of primer sequence   cycles
CEL16 Endo-1,4-beta-D-glucanase 112 B. napus AJ242807.1 147 5‘-GGCTTCTGCATCCATTGTCT-3‘ 45
  (cellulase)     258 5‘-TGCACTGTATCTGCCTCTCCT-3‘  
FBP Fructose-1,6-bisphosphatase 111 B. napus mRNA AF081796.1 81 5‘-GCCTCATCTCAGCCACAAAT-3‘ 45
  precursor     191 5‘-AACCGCCATACACCTCACTC-3‘  
SPS Sucrose-phosphate synthase 106 B. rapa subsp. AY184484.1 194 5‘-CAGATGGGAACGAGGAACAT-3‘ 45
    pekinensis mRNA   299 5‘-CTCGCAAGGGCAAGTATCAT-3‘  
RBC Ribulose 1,5-bisphosphate carboxy- 96 B. napus mRNA X07367.1 378 5‘-ACTACGATGGCCGTTACTGG-3‘ 45
  lase/oxygenase small subunit     473 5‘-CCGTTTTGCACTCTTGGACT-3‘  
PK Pyruvate kinase 89 B. napus mRNA DQ539628.1 22 5‘-CCAAGGTTGTGGTGCTGAT-3‘ 45
      110 5‘-CTGATGCGGTGATAATGGAAT-3‘  
PECT Ethanolamine-phosphate 192 A. thaliana mRNA NM_129424.4 483 5‘-CTGTGAAGTGGGTGGATGAA-3‘ 45
  cytidylyltransferase     674 5‘-CAGTGCGCTTAATCTGCTTG-3‘  
APL Glucose-1-phosphate adenylyl- 195 A. thaliana mRNA NM_121927.3 581 5‘-CACTCAAACGCCAGGAGAAT-3‘ 55
  transferase large subunit 1     775 5‘-CTTATATCCGCGCCACTCTG-3‘  
AILP1 Aluminum-induced protein 123 B. napus mRNA JCVI_24 663 5‘-CTTGCTAAAAGGGGCTTGTG-3‘ 45
      785 5‘-GCAGGAATGGCAGTGATCTT-3‘  
GER1 Germin-like protein subfamily 3 189 B. napus mRNA JCVI_391 385 5‘-ATCACCGCTGGATTCATCTC-3‘ 45
  member 1     573 5‘-AGCAAATAGCGCAAAGTCAAG-3‘  
NOI nitrate-induced domain protein 198 B. napus mRNA JCVI_10152 249 5‘-CCAGCATCAGCAGAAGGTTT-3‘ 45
      446 5‘-TTGTGTCCGTCAAGAGTCCA-3‘  
MyAP myrosinase-associated protein 131 B. napus mRNA JCVI_1353 34 5‘-AAAATGGCACCCAACTTCAG-3‘ 45
      164 5‘-TTGGAATCTCCGAATGTGAAC-3‘  
GRF1 Growth-regulating factor 1 101 B. napus mRNA JCVI_41561 121 5‘-CTTGGAGTTCGGTTTGAAGG-3‘ 45
      221 5‘-CCGGATCTTTCTTGCTTGTT-3‘  
VPS2 ESCRT c III complex 123 B. napus mRNA JCVI_31511 148 5‘-TTTGCACCAGCAAGAGAGG-3‘ 45
      270 5‘-GGCTTGTTTCATGTGTGACG-3‘  
UBP15 Ubiquitin carboxyl-terminal 117 B. napus mRNA JCVI_5013 676 5‘-TGAGAGGCAACTGGTTCAGA-3‘ 45
  hydrolase 15     792 5‘-TTAGAGGACGCGGATACGAT-3‘  
GF14 G box factor 14-3-3 omega 145 B. napus mRNA JCVI_22791 621 5‘-TTGCCCATTCGCTTTTATTC-3‘ 45
  protein     765 5‘-AAGGTTCGATGGCAGACACT-3‘  
ACT Actin 104 B. napus GQ339782 156 5‘-TCAGGCCGTTCTTTCTCTTTAC-3‘ 45
      259 5‘-GAGCATAACCCTCGTAGATTGG-3‘  
  1. aAbbreviation.
  2. bOrganism of the used reference sequence.
  3. cEndosomal sorting complex required for transport.