Figure 6

Sequential localization of the anti-BrCENH3 antibody and centromeric repeats on radish. (a) RsCENH3 localization at mitotic metaphase chromosomes of radish; (b) The same cell was hybridized with CL1; (c) Merged fluorescence signals from a and b; (d) Merged fluorescence signals from c and chromosomes; (e) RsCENH3 localization at mitotic metaphase chromosomes of radish; (f) The same cell was hybridized with CR-Radish; (g) Merged fluorescence signals from d and e; (h) Merged fluorescence signals from g and chromosomes; (i) CR-Radish localization at mitotic metaphase chromosomes of radish; (j) The same cell was probed with CL1; (k) Merged fluorescence signals from g and h; (l) Merged fluorescence signals from h and chromosomes. Bars = 5 μm.