Figure 6

qPCR of miRNA target gene expression analysis in LPS-elicited callus tissue (A) and leaf tissue (B). Treated samples (C3 and L3) showed differential gene expression relative to control samples (C0 and L0 respectively). The data was normalized using elongation factor 1-alpha and actin 8 to give the relative gene expression wherein error bars represent the standard error of mean. Expression analysis was performed on three biological repeats with three technical replicates of each. (a) Indicates no significant differences, with P > 0.05, (b) indicates that there was a significant difference with P < 0.05, (c) indicates that there was a highly significant difference with P < 0.01. Auxin response factor 10 (AUX), Concanavalin A-like lectin protein kinase (LEC), Copper superoxide dismutase (CDS), Nuclear factor Y subunit A8 (NFY), Growth regulating factor 4 (GR), Myb domain protein 101 (MYB101), Plantacyanin (PCYA), Receptor-like protein kinase (RLK), Ubiquitin-protein ligase (UBC) and Squamosa promoter-binding-like protein (SPL).