inactivation does not affect embryo development. Histochemical staining of DAG2:GUS during embryogenesis, in early globular, globular, heart, late heart, torpedo and mature embryo (A).Relative expression level of DAG2 in wild-type seeds at 13, 16 and 19 Days After Pollination (DAP), and in mature seeds (28 DAR). Relative expression levels were normalized with that of the UBQ10 gene, and are presented by the ratio of the corresponding mRNA level in mature seeds, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05) (B). Phenotypes of wild-type (a, c) and dag2 mutant (b, d) embryos, at globular (a, b) and heart stage (c, d) (C).