Figure 4

DAG2 expression is repressed by PIL5. Relative expression level of DAG2 in pil5 mutant and wild-type seeds. Seeds were imbibed 12 hours in the dark (D), or under R light (R). Relative expression levels were normalized with that of the UBQ10 gene, and are presented by the ratio of the corresponding wild-type mRNA level in D, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05).