Figure 3

DAG2 is directly regulated by DAG1. Relative expression level of: DAG2 in dag1 mutant and wild-type (WT) seeds (A), and of DAG1 in dag2 mutant and wild-type seeds (B). Seeds were imbibed 12 hours in the dark (D), or under R light (R). Relative expression levels were normalized with that of the UBQ10 gene and are presented by the ratio of the corresponding wild-type mRNA level in D, which was set to 1. Similar results were obtained from three independent experiments, and a typical result is presented with SD values. Significative differences were analyzed by t-test (*P ≤ 0,05). (C) Graphic representation of the DAG2 promoter. Underlying thick lines marked by letters (a, b, c, d) are referred to different promoter fragments used for qPCR, containing 0 (a, b), 4 and 7 Dof sites respectively (c,d). (D) Chromatin from dag1DAG1-HA seeds was immunoprecipitated with anti-HA or without antibody, and the amount of DNA was measured by qPCR. B3 is referred to the positive control, fragment B3 of the AtGA3ox1 promoter bound by DAG1-HA The values of fold enrichment are the average of three independent experiments presented with SD values. Significative fold enrichment was analyzed by t-test (*P ≤ 0,05).