35S:NTAP:AtCTF7∆B plant morphology changes in later generations. (A) Wild type Columbia plant. (B-E) Morphological alterations get progressively worse in 35S:NTAP:AtCTF7∆B plants through self-pollination. (B) Second generation plants are normal, but reduced fertile. (C) Both dwarf and non-dwarf, reduced fertile plants are observed in third generation plants. (D) Fourth generation plants. (E) Sixth generation plants, showing a higher frequency of dwarf plants. Defects such as reduced apical dominance (arrow) and phyllotaxis disturbances (asterisks) are observed. (F-I) Representative 35S:NTAP:AtCTF7∆B dwarf plants. Inflorescence defects include acaulescent (G), multiple inflorescence branches at the first node (H) and no inflorescence (I). Leave defects include aberrant rosette size and shape (F, H and I). (J)
atctf7-1 plant showing an early senescence phenotype (arrow). Plants B to I are from Line 11. All plants are grown under the same conditions. Plants in A-H and J are approximately 30 days old; plant in I is 40 days old. Scale bar = 5 cm in A-E and 2 cm in F-J.