Figure 6
Light microscopy of cross-sections of T 1 35S:HvCesA4 and 35S:HvCesA8 stem internodes stained with Toluidine Blue. Equivalent internodes were sectioned using vibratome (~30-50 μM thick) from (A, B) wild-type or null, (C, D) dwarfed 35S:HvCesA4 transgenic T1 plants and (E, F) 35S:HvCesA8 transgenic T1 plants. Red arrows indicate xylem vessels and in D, they are collapsed and irregular in shape. Scale bars denote 100 μM. E = epidermis, VB = vascular bundle, Ph = phloem tissue, Xy = meta-xylem, BS = bundle sheath, PC = parenchyma cells.