PsOep23 shows channel activity. Representative current traces of a bilayer containing a single active PsOep23 channel. Holding potential is set to +100 and −100 mV, respectively. The channel activity is characterized by short gating events from the open (o-main) to the sub-conductance state (o-sub). Some events are faster than the time resolution of the measurement which becomes apparent in the current histogram and a zoom into the trace. The electrolyte solution contained 250 mM KCl, 50 mM Mops/Tris pH 7.0 (symmetrical cis/trans).