Figure 3

Effect of NaSA on DSF biosynthesis and signaling. (A), Expression of DSF biosynthesis and responsive genes rpfF, rpfC, rpfG, and adhesin-encoding pilA in XKK12 WT (pPIP122) grown in PY broth supplemented or not with 1 mM NaSA. Data are means ± SE of two technical and two biological replicates. Asterisks indicate statistically significant differences compared to the control (T-test: n = 4; α = 0.05). (B), Quantification of DSF and BDSF produced by XKK12 WT (pPIP122) grown in PY broth containing 0 or 1 mM NaSA. Data are means ± SE of three independent experiments. Asterisks indicate statistically significant differences compared to the control (T-test: n = 3; α = 0.05).