Figure 1

NaSA suppresses basal and DSF-induced swimming motility of Xanthomonas oryzae pv. oryzae ( Xoo ) strain XKK12 (pPIP122). (A) and (B) Various concentrations of NaSA were added to swimming plates prior to inoculation with 3 μl XKK12 WT (pPIP122) suspension (10⁹ CFU/ml). The plates were incubated for four days at 25°C and evaluated by measuring the diameter of the swimming zone. Data are means ± SE. Different letters indicate statistically significant differences (Mann-Whitney: n ≥ 9; α = 0.05). (C), Phenotype of XKK12 WT (pPIP122) on swimming plates containing 0 (left) or 1 mM NaSA (right). (D), Effects of 1 mM NaSA, 2 mM NaSA and/or 3 μM DSF on the swimming of XKK12 WT (pPIP122) and oryR knockout mutant (pPIP122) (oryR ⁻) bacteria. Data are means ± SE. Different letters indicate statistically significant differences (Mann-Whitney: n ≥ 9; α = 0.05).