Table 3 qRT PCR primers designed for amplification of products from 80-120 bp
Target sequence | Primer sequence | Product size in bp |
|---|---|---|
CvDisease resistance protein [contig02315] | Forward: GAAGTACAACGGAAGCACGA | 106 |
Reverse: CCTCTAGCAAACCGGAAAAG | ||
CvTATA binding [contig05402] | Forward: AACATCGTTGGTTCCTGTGA | 101 |
Reverse: CCAGGAAATAGTTCGGGTTC | ||
CvTSa [contig04235] | Forward: GTGCTCTTGGTTGGTTGTGA | 89 |
Reverse: ACAGGCATGGTCGTCTTTTC | ||
Cv18S rRNA, [GenBank: AF419791] | Forward: AGGGTTGAGGCAGAGAGAGA | 117 |
Reverse: AGAACCCCACAGAACCTCAG | ||
CvActin [contig03212] | Forward: GCATCACTAAGCACCTTCCA | 111 |
Reverse: CCCTCATCACGCAATTTAGA | ||
CvAP3 [contig09453] | Forward: ACATCAGTCCCCCTTCTACG | 88 |
Reverse: CATAGTGCGAGCTCCAAAGA | ||
CvPI [contig07420] | Forward: CCCAATTTGCAGGATAGGTT | 93 |
Reverse: TCCCCATTACAGTTCCAACA |