Figure 5
Intrinsic flavins and H 2 O 2 in riboflavin-fed and water-treated plants. Water or an aqueous solution of riboflavin was used to immerse seeds and treat 10-day-old plants by spraying over plant tops. Analyses for flavin concentrations (a), subcellular H2O2 distribution (b), and H2O2 concentrations (c) were performed on the two youngest leaves of 12-day-old plants. Quantitative data shown are average values ± standard deviations based on three experimental repeats each containing 15 plants. On bar graphs, different letters in regular and italic fonts indicate significant differences by analysis of variance using Fisher’s least significant difference test and Tukey-Kramer’s test, respectively (n = 3; P < 0.01).