Figure 7

Analysis of enhancer activity in transgenic maize plants. The relative locations of the activation tagging element and the gene examined are shown. The activation tagging element is represented by four yellow boxes representing the 4× SCBV enhancer tandem array and the blue arrow representing the AAD1 selectable marker cassette. Maize genes are represented by the green box with the transcription start site and direction represented by the arrow. Distance to promoter reflects the estimated distance from the enhancers to the translational start site of the gene. The relative transcript abundance is the amount of transcript of the gene in transgenic plants compared with the level of the transcript in non-transgenic plants. An asterix indicates that the values were significantly different as determined by a t-test (α = 0.05).