Figure 1

Comparison of the SCBV-IM and ZmUBI1 promoters in transgenic maize tissues. Transgenic, single copy maize events containing SCBV-IM::AAD1 and ZmUBI1::AAD1 constructs were analyzed for AAD1 transcript accumulation in the youngest fully expanded leaf of V3 (A) and V8 (B) stage plants, the leaf just below the ear of R1 plants (C), a 1 cm section from the tip of the root in V3 (D) and V10 (E) stage plants, and in the tassels of R1 stage plants (F). Three events for each of the two constructs were compared by RT-qPCR using primers specific to the AAD1 transcript and normalized to an endogenous transcript, TIP (for leaf and tassel tissues) or MAZ95 (for root tissues). The error bars represent the standard deviation of three measurements. Analysis of Variance (α = 0.05) indicate that more AAD1 transcript accumulate in the SCBV-IM::AAD1 events than in ZmUBI1::AAD1 events in leaves of V3 stage plants, the roots of V3 and V8 stage plants, while similar levels of AAD1 transcript accumulate in leaves of V8 stage plants. ZmUBI1::AAD1 transgenic plants accumulate more AAD1 transcript than SCBV-IM::AAD1 transgenic plants in leaf and tassel tissues at R1.