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Table 4 Confirmation of miRNA:target pairs by RLM-RACE

From: Potential functions of microRNAs in starch metabolism and development revealed by miRNA transcriptome profiling of cassava cultivars and their wild progenitor

miRNA

Target

Annotation

Cleavage sites

Upstream

Complementary region

Downstream

miR156/

miR535

cassava4.1_006419m

SPL2

/

10th/11th(8), 11th/12th(3)

−35th/–36th(1)

cassava4.1_009657m

SPL13b

/

9th/10th(2), 10th /11th(4), 11th/12th(1)

−1st/–2nd (1)

miR159/

miR319

cassava4.1_004606m

MYB33

/

11th/12th(10)

−52th/–53th(1),

–115th/ –116th(1)

cassava4.1_030321m

MYB81

/

11th/12th(6)

−59th /–60 th(1)

miR394

cassava4.1_021267m

APL2

+18th(1), +35th(3), +42th(1), +69th(2)

2nd(3), 14th(1)

−27th(1), −29th(1), −49th(1), −92th(1), −105th(1), −109th(1), −139th(1)

cassava4.1_007038m

F-box

/

10th(2)

−31th(1), −52th(1), −55th(1), −59th(1), −68th(1), −75th(1), −102th(1)

miR160

cassava4.1_002668m

ARF10

/

11 th(10)

/

miR167

cassava4.1_009942m

RD19

+5th(1), +25th(1), +145th(1), +196th(1)

 

−41th(1)

miR169

cassava4.1_011576m

NF-YA3

+30th(1), +6th(1)

11th(5), 15th(1)

/

miR396

cassava4.1_003731m

GRF1

+69th(2)

6th(1), 11th(6)

−45th(1), −60th(1), −188th(1)

miR398

cassava4.1_024493m

DIR-like

+8th(10)

/

/

miR477

cassava4.1_008074m

CLB

+37th(8)

/

/

miR166

cassava4.1_009671m

GH17

/

/

−8th(3), −16th(6), −34th(1)

new-21

cassava4.1_006393m

EDR2

/

/

−112th(6), −160th(2), −176th(1), −177th(1), −178th(1), −182th(1)

  1. Note: As an example, 10th/11th (8) indicate that there were eight cleavage sites at the 10th or 11th nucleotide in the complementary region of cassava4.1_006419m and miR156/535; alternatively, −35th/–36th (1) indicates that there was one cleavage site downstream of the complementary region of cassava4.1_006419m and miR156/535.