Table 1 Primers used in this study
From: Overexpression of tomato SlNAC1transcription factor alters fruit pigmentation and softening
Primer name | Sequence | Purpose |
|---|---|---|
NAC1F | 5′-GGAAATGAACAAAGGAGC-3′ | Amplification of SlNAC1 |
NAC1R | 5′-GTCATGGATCACACTCAA −3′ | Amplification of SlNAC1 |
NAC1F’ | 5′-GGCTTGATGATTGGGTATTGTG-3′ | qRT-PCR of SlNAC1 |
NAC1R’ | 5′-GCTTGTAGTTTCCTTGTTGTCC-3′ | qRT-PCR of SlNAC1 |
EF-1αF | 5′-GGAACTTGAGAAGGAGCCTAAG-3′ | qRT-PCR of EF-1α |
EF-1αR | 5′-CAACACCAACAGCAACAGTCT-3′ | qRT-PCR of EF-1α |
230.2 F | 5′-AAGGCTGGACGATTGGGTTCTATG-3′ | qRT-PCR of Solyc06g060230.2 |
230.2R | 5′-ATTGCTGCGGCTGAGGATGTG-3′ | qRT-PCR of Solyc06g060230.2 |
410.2 F | 5′-TCATCATCGTCATCGTCATCTCAGT-3′ | qRT-PCR of Solyc07g063410.2 |
410.2R | 5′-TCCCGCCATAGCAGCCCAAT-3′ | qRT-PCR of Solyc07g063410.2 |
470.1 F | 5′-GGCGGTGAGTGAAGGTGATGTAA-3′ | qRT-PCR of Solyc11g017470.1 |
470.1R | 5′-GCTGGAATCGGCGTGAAGTT-3′ | qRT-PCR of Solyc11g017470.1 |
620.1 F | 5′-GGCAATTCTCGCTGGGCTCAA-3′ | qRT-PCR of Solyc12g013620.1 |
620.1R | 5′-GTTGTTGTCGCTGTGAATGTGGTT-3′ | qRT-PCR of Solyc12g013620.1 |
PSY1F | 5′-GCATCATATATTACCCCGGCAG-3′ | qRT-PCR of SlPSY1 |
PSY1R | 5′-TCGGACAAAGCACCATCGA-3′ | qRT-PCR of SlPSY1 |
LCYbF | 5′-TACCAATGGGTGGTCCACTTC-3′ | qRT-PCR of SlLCYb |
LCYbR | 5′-CCTTGCCACCATATAACCGGT-3′ | qRT-PCR of SlLCYb |
LCYeF | 5′-ATGGATGTGGCAGGGATTTC-3′ | qRT-PCR of SlLCYe |
LCYeR | 5′-CTTTTCTCATGTCATTTGGTGCA-3′ | qRT-PCR of SlLCYe |
CYCBF | 5′- GGCTCAATTCGACGTGATCA-3′ | qRT-PCR of SlCYCB |
CYCBR | 5′- AGAGTGGTGAAGGGTCAACACA-3′ | qRT-PCR of SlCYCB |
ACS2F | 5′-AAGCTTAACGTCTCGCCTGG-3′ | qRT-PCR of SlACS2 |
ACS2R | 5′-CCACCCTGGCTCTTGACATT-3′ | qRT-PCR of SlACS2 |
ACS4F | 5′-TCAACGTCTCCCCTGGATCT-3′ | qRT-PCR of SlACS4 |
ACS4R | 5′-TGCAAGTGCGATCTCCATTG-3′ | qRT-PCR of SlACS4 |
ACO1F | 5′-TAATGGGAATGGGAAGAAAAGATT-3′ | qRT-PCR of SlACO1 |
ACO1R | 5′-ACAAAGCAAGATAAAGCACCCC-3′ | qRT-PCR of SlACO1 |
DBDF | 5′-GGATCCACGGTGAAAAAGAGTGGTA-3′ | Cloning the DBD of SlNAC1 |
DBDR | 5′-CTCGAGAGTTATTGTTCTTGCCAGCAG-3′ | Cloning the DBD of SlNAC1 |
PSY1PF | 5′-GGTACCGGAGTTAGAGGGTAAGTTAC-3′ | Cloning the promoter of SlPSY1 |
PSY1PR | 5′-CTCGAGACACAGACCATAGCTCTACC-3′ | Cloning the promoter of SlPSY1 |
ACS2PF | 5′-GGTACCCTTTCTCACGTGTAGCTTC-3′ | Cloning the promoter of SlACS2 |
ACS2PR | 5′-CTCGAGTACGCATTAAAAGAAGATCTACG-3′ | Cloning the promoter of SlACS2 |
ACO1PF | 5′-GGTACCCGTGGTCTTTCGAGGTTTGC-3′ | Cloning the promoter of SlACO1 |
ACO1PR | 5′-CTCGAGGACGTAAACATAAGAAATAGC-3′ | Cloning the promoter of SlACO1 |
NCED1F | 5′-AGGCAACAGTGAAACTTCCATCAAG-3′ | qRT-PCR of SlNCED1 |
NCED1R | 5′-TCCATTAAAGAGGATATTACCGGGGAC-3′ | qRT-PCR of SlNCED1 |
NCED2F | 5′-TGGTTTTCATGGGACATTCATTAGC-3′ | qRT-PCR of SlNCED2 |
NCED2R | 5′-ATCTCCCTTCTCAACTCCCTATTCC-3′ | qRT-PCR of SlNCED2 |
PGF | 5′-AAGCATGGAATGAAGCATGTTCATCTAG-3′ | qRT-PCR of SlPG |
PGR | 5′-CAAAAGCAATCCAAAGCCTTCTATC-3′ | qRT-PCR of SlPG |
Exp1F | 5′-AATCAAATGCGGTTTTAACTGGTCAAT-3′ | qRT-PCR of SlExp1 |
Exp1R | 5′-AATCAAATGCGGTTTTAACTGGTCAAT-3′ | qRT-PCR of SlExp1 |
Cel1F | 5′-AGTTGCCTCTGAGTTTAGTTGGGATG-3′ | qRT-PCR of SlCel1 |
Cel1R | 5′-TCCACCTGGGGTTGTCTTAATTTGTA-3′ | qRT-PCR of SlCel1 |
Wiv1F | 5′-GTGCTGGAGGAAAAACGTGC-3′ | qRT-PCR of SlWiv1 |
Wiv1R | 5′-GATCGTCTCTGCGCCATTGT-3′ | qRT-PCR of SlWiv1 |