Figure 4

Physiological evaluation of transgenic plants under water deficit. Predawn leaf water potential (A), net CO₂ assimilation rate (B), stomatal conductance (C) and transpiration rate (D) of transgenic B1 and B3 lines and wild-type (WT) plants grown under two water regimes. Water deficit was imposed at R₃ stage (beginning of pod formation). Measurements were recorded at one, six and twelve days after imposing water stress. Asterisks indicate significant differences of B1 or B3 lines compared to wild-type plants in each evaluation day and water regime (Student’s t-test, *: p < 0.05, **: p < 0.01). Values are means ± SD (n = 5).