Validation of pCAMBIA-derived CRISPR/Cas binary vectors in Arabidopsis . (A) Physical map of T-DNA of the pCAMBIA-derived vector carrying two-gRNAs targeting two Arabidopsis genes (CHLI1 and CHLI2). The alignment of gRNA with its target gene is shown. Only aligned regions of interest are displayed. -rc, reverse complement. (B) Phenotypes of all transgenic seedlings from one screening. The T0 seeds were screened on hygromycin MS plates for 7 days, and all of the hygromycin-resistant seedlings were transferred to a fresh MS plate before photographing. The albino seedlings were numbered.