Effects of sucrose treatment on general and specific translation in the light and in the dark. A) Absorbance profile of sucrose density gradients. Fractions collected for microarray analysis marked on top. B) Polysomal areas as percentage of total area under the curves in different treatments, displayed as difference with the corresponding control. The asterisks mark significant differences (p < 0.05). Average of 14 biological replicates, ±SD. C) Number of genes affected by sucrose treatment in the dark or in the light compared to their corresponding control on the transcriptional (steady-state mRNA) level (SL), their polysomal mRNA levels (PL) or their polysomal occupancy (RO, PL/T). D) Number of genes affected by dark (sorbitol) treatment compared to the light (sorbitol) control. E) Plot showing the polysomal occupancy vs. transcriptional changes induced by sucrose in the light compared to the control. Red dots mark genes with significantly differential ribosomal occupancy.