Figure 1
From: Rice SUV3 is a bidirectional helicase that binds both DNA and RNA
ATPase activity of purified OsSUV3 in presence of DNA and RNA. (A) ATPase activity of purified OsSUV3in the presence of ssDNA. Lanes 1-7, reactions with different concentration (10-240 nM) of enzyme. (B) Time dependence of ATPase activity of OsSUV3 (180 nM) in the presence of ssDNA. Lanes 1-7, reactions with enzyme at various time points. (C) ATPase activity of purified OsSUV3 in presence of RNA. Lanes 1-7, reactions with different concentration (10-240 nM) of enzyme. (D) Time dependence of ATPase activity of OsSUV3 (180 nM) in presence of RNA. Lanes 1-7, reactions with enzyme at various time points. Lane C in A-D shows reaction without enzyme. The quantitative data of the autoradiograms in A-D gels are shown as histograms on the right side of each autoradiogram.