Figure 2

Chromatograms of scopoletin standard and methanol extracts from Arabidopsis thaliana roots. The column effluent was monitored with fluorescence detector with excitation at 340 nm and emission at 460 nm. The peak for glucoside of scopoletin – scopolin (scl); the peak for scopoletin (sct). (A) Chromatogram of scopoletin standard. (B) Chromatogram of methanol extract from Arabidopsis roots before enzymatic hydrolysis. (C) Chromatogram of methanol root extract subjected to hydrolysis using β-glucosidase. The peak for scopoletin is a dominant peak of total chromatogram.