Table 4 Primers for qRT-PCR to verify above seven genes involved in catechins biosynthesis in tea plant
Target CDSs | Primer | Sequence (5′ to 3′) |
|---|---|---|
Tea_T4_Unigene_BMK.28458 (LAR-1) | F | AAACTCTTCAAGACAAAGGCGCTAA |
R | TCTATCAATCGCCGCACCCTC | |
Tea_T4_Unigene_BMK.50436 (LAR-2) | F | GACTGTAGCAGCAGAAGTAGCC |
R | TCAATCTTATGGTCCCTCAAA | |
Tea_T4_Unigene_BMK.41423 (LAR-3) | F | GCTGTGGGTGGTGCTAAT |
R | GCGATCCAAAGGAGGAAT | |
Tea_T4_Unigene_BMK.49588 (ANS-1) | F | ATGACTACAGTGGCTGCCCCGA |
R | CAACGCCTCCCGACACCTCTC | |
Tea_T1_Unigene_BMK.79499 (ANS-2) | F | ACGAGGGCAAATGGGTCA |
R | CCTTATTAACGAGTCCACGATG | |
Tea_T1_Unigene_BMK.72783 (ANR-1) | F | CTGTCCGAGACCCAGGCAATC |
R | GGGCGTCAAAGCTCTGTTCAT | |
Tea_T4_Unigene_BMK.60174 (ANR-2) | F | CAATGGCAATGGTAACAACA |
R | TCCAGTGCTACGAGGTGAG | |
TaGAPDH (internal control gene) | F | TTGGCATCGTTGAGGGTCT |
R | CAGTGGGAACACGGAAAGC |