Figure 3

Methylation of crucial sites of β-1,3-glucanase gene. The analysis of the methylation of two sites in exons and one site in the intron of flax treated with OLIGOs (B1, B2, B3 and B4) at 24 h and 48 h after exposure to OLIGOs in comparison with control, non-treated flax (C) and in EMO-βGlu flax (EB) in comparison with control flax from field (Cf) was determined by digesting genomic DNA with restriction enzymes HpaII-MspI with subsequent semi-quantitative PCR reaction, the separation of PCR product on agarose gel and the quantification of the bands by densitometry. Data represent the mean ± standard deviations from three independent experiments. The significance of the differences between the means was determined using Student’s t test (*P < 0.05, **P < 0.01).