Motility of GFP-RabA1d vesicles in growing root hairs. Kymograph showing the motility of the GFP-RabA1d vesicles in root hairs (A). Estimation of the average speed of the GFP-RabA1d vesicles in root hairs treated with acto-myosin inhibitors, latrunculin B (LatB) and butane-dione-monoxime (BDM), respectively (B). Mobility and distribution of small RabA1d-positive TGN vesicles in diverse root tissues observed by light-sheet microscopy (C-E). 3D reconstruction of a stack of images (C). Single sections at different z-depths ((1): z = 0 μm, (2): z = 16.5 μm, (3): z = 36.8 μm, (4): z = 50 μm) are depicted in (D). The dashed boxed area is enlarged in (E). Bars represent 20 μm in D and 5 μm in E.