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Figure 1 | BMC Plant Biology

Figure 1

From: Diverse functional evolution of serine decarboxylases: identification of two novel acetaldehyde synthases that uses hydrophobic amino acids as substrates

Figure 1

HPLC-EC detection of indole-3-acetaldehyde generated in MtAAS and tryptophan reaction mixtures. (Chromatograms A-F) Y-axis represents the output in microamps and the x-axis represents retention time. Chromatograms (A-C) illustrate the indole-3-acetaldehyde (the major broad peak) formed in MtAAS and tryptophan reaction mixtures after 5 min, 20 min and 40 min incubation, respectively. Chromatograms (D-F) illustrate the indole-3-ethanol (tryptophol) formed in borohydride reduced MtAAS and tryptophan reaction mixtures after 5 min, 20 min and 40 min incubation, respectively. Chromatogram (G) shows the detection of authentic indole-3-ethanol standard.

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BMC Plant Biology

ISSN: 1471-2229

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